Abstract
AbstractThe microneutralization test is a sensitive and specific technique used for quantitation of virus-specific neutralizing antibodies in serum samples of infected or vaccinated chickens. Because of the characteristic of viral strictly cell tropism, the neutralization test for infectious bronchitis virus (IBV) is generally based on chicken embryos, tracheal organ cultures, and primary chicken kidney (CK) cells. In recent years, we have serially passaged the H120 vaccine strain in CK cells and obtained a Vero-cell-adapted strain HV80. Based on this strain, we developed a new neutralization test for anti-IBV antibody detection using Vero cells. In addition to having the advantages of neutralization assay, compared with the use of chicken embryos and primary tissue cells, the microneutralization test using Vero cells is easy to perform, does not need special equipment, and is suitable for use with large quantities of serum samples.Key wordsInfectious bronchitis virusNeutralization testTitration
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