Abstract
Nano-structured and micro/nano-hierarchical structured TiO2 coatings were produced on polished titanium by the micro-arc oxidation (MAO) technique. This study was conducted to screen a suitable structured TiO2 coating for osteoblast adhesion and differentiation in dental implants. The formulation was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and wettability testing. Adhesion, proliferation and osteogenic differentiation of MG63 cells were analysed by SEM, Cell Counting Kit-8 (CCK-8) and quantitative real-time PCR. The micro/nano-hierarchical structured TiO2 coating with both slots and pores showed the best morphology and wettability. XRD analysis revealed that rutile predominated along with a minor amount of anatase in both TiO2 coatings. Adhesion and extension of MG63 cells on the micro/nano-hierarchical structured TiO2 coating were the most favourable. MG63 cells showed higher growth rates on the micro/nano-hierarchical structured TiO2 coating at 1 and 3 days. Osteogenic-related gene expression was markedly increased in the micro/nano-hierarchical structured TiO2 coating group compared with the polished titanium group at 7, 14 and 21 days. These results revealed the micro/nano-hierarchical structured TiO2 coating as a promising surface modification and suitable biomaterial for use with dental implants.
Highlights
Osseointegration is defined as the direct bonding of living bone tissue with surgical implants that can replace bone and perform load-bearing functions [1]
Osteogenicrelated gene expression was markedly increased in the micro/ nano-hierarchical structured TiO2 coating group compared with the polished titanium group at 7, 14 and 21 days. These results revealed the micro/nano-hierarchical structured TiO2 coating as a promising surface modification and suitable biomaterial for use with dental implants
The microstructure was characterized by scanning electron microscopy (SEM) in the Ti, 9%-2MAO and 11%-13MAO groups, as shown in figure 1
Summary
Osseointegration is defined as the direct bonding of living bone tissue with surgical implants that can replace bone and perform load-bearing functions [1]. Osteoblast adhesion and differentiation on implant surfaces are two important indices affecting osseointegration [2]. Bone formation mainly depends on the surface characteristics of surgical implants [3]. The surface chemical properties of implants are critical for early bone formation [5]. Enhanced surface energy and wettability can stimulate the interaction between the implant surface and its surrounding biological environment. A hydroxylated or hydrated surface, which exhibits immediate wettability, contributes to the production of a more differentiated osteoblast phenotype [6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
