Micromorphology and monolignols of leaf epidermis in Phragmites australis (Poaceae) of air-aquatic and terrestrial ecotypes.

Abstract

The microstructure of leaf epidermis, the localization, and content of syringyl (S) and guajacyl (G) monolignol in epidermal cells of Phragmites australis grown in water and moderate drought soil on the bank of the Venetian strait of the Dnipro River in Kiev (Ukraine) were studied with the electron microscopic method and laser confocal microscopy. The trichomes, guard cells of stomata, epidermal cells of vaulted zones over veins of adaxial and abaxial surface contained syringyl and guajacyl, the content of which depended on plant ecotype. We established that moisture soil influenced the leaf epidermis ultrastructure, on the change of monolignols content and S/G ratio in epidermis leaf of reed. The investigations show that trichomes and the cells of the vaulted zone in the leaf epidermis were the main accumulator of monolignols. The obtained results suggest that change of the microstructure of leaves, content of the monolignols, and S/G ratio in leaves contribute to the preservation of optimal water status of plants and can be considered as the signs of plant phenotypic plasticity and adaptive marker depending on the conditions of water supply.