Micromorphological studies of the leaf and stem of Camellia sinensis (L.) Kuntze with reference to their taxonomic significance.

Abstract

The micro-morphological examinations of the leaf lamina, petiole and stem for Camellia sinensis (L.) Kuntze (Theaceae) was carried out using a variety of microscopic techniques widely implemented in the area of medicine. The objective of this study was the micromorphological characterization of stem, petiole, lamina, stomata, leaf trichomes and other internal characters. The anatomical studies included the cross section of stem and leaf of Camellia sinensis thereby exhibiting a typical pattern of arrangement of tissues similar to woody plants. Some idioblastic sclereids like astrosclereids, osteosclereids were found in the medullary parenchyma of tea stem and leaf. Large numbers of sclereids were found mainly in the parenchymatous tissue of leaf petiole. Other micro-morphological features like trichomes, stomata, and different tissue layer were also recorded. The leaf trichomes were unicellular, long and densely present in the lower surface of immature leaf but a decrease in amount of trichomes was seen in the mature leaf making it a prime taxonomic feature of the tea leaf. The microscopic morphological analysis of the stem, petiole, lamina, stomata, leaf trichomes of Camellia sinensis can be used for its identification. In addition, these techniques can be further implemented for the taxonomic characterization thereby establishing a genetic relationship and solving taxonomic disputes in the field of plant systematics.