Abstract
In biological research, various experiments such as behavioral experiments and physiological ones are often conducted with pharmacologically treated animals. In such experiments, it is necessary to inject the same volume of solution into numerous small animals, such as insects to prepare several experimental subjects. However, repeating manual injections is burdensome, and it is also difficult to maintain injection quality and consistency. We have developed a microinjection system that can support and semiautomate the injections of small animals. The system consists of two cameras, a micromanipulator, a syringe pump, and a structural framework all operated from a personal computer to quickly inject the same volume of liquid solutions at the same position and depth into small animals. The microinjection system has sufficient extensibility for it to be used in a variety of applications.
Highlights
Microscale injection support system Biological Sciences Biological sample handling and preparation Various USD 3800 + USD 12000(uMP manipulator system) https://osf.io/3mtxk/
Several microinjection systems have already been developed, most have been for injection into cells [4,5,6] or very small animals such as C. elegans [7]
The following is a brief summary of the contents of the files: universal_stage.zip Computer-aided design (CAD) files required for universal stage, including Autodesk Inventor part file and stereolithography (STL) file required for 3D printing. machining_drawing.zip CAD files required for camera and manipulator adapters, including Autodesk Inventor part file and mechanical drawing. msis2_cad.zip CAD files indicating the positional relationship of 3D structural framework, cameras, and manipulator, including Autodesk Inventor part file. needle_holder_cad.zip CAD files required for needle holder operation, including Autodesk Inventor part file and mechanical drawing. injectionTimer_pcb_design.zip Eagle design files required for injection timer printed circuit board (PCB). msis2.zip C++ source codes for controlling the whole system
Summary
Various biological research experiments, such as behavioral and physiological experiments, are typically conducted with pharmacologically treated animals Such experiments are being conducted on insects [1]. Because it is quite common to inject liquid solutions into small animals such as insects, such a system is useful for our project and for other biological experiments; the development of such a system should have a significant ripple effect. We have designed and developed a microinjection support system for small biological subjects such as insects. The design requirement of the system are as follows; (1) The system must be able to inject a certain volume of solution at the same position and depth into small creatures such as insects. The design requirement of the system are as follows; (1) The system must be able to inject a certain volume of solution at the same position and depth into small creatures such as insects. (2) The system must be able to efficiently inject biological subjects in almost the same length of time as manual injections; that is, several minutes per injection target. (3) The system must show sufficient extensibility for it to be used in our project and in other micromanipulations including microinjections
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