Microinjection Into the Lateral Ventricle: Validation of the Procedure in Estrogen‐Treated Ovariectomized Cynomolgus Monkey

Abstract

To demonstrate that microinjection into the lateral ventricle intracerebroventricular (i.c.v.) of the brain of monkeys does not perturb normal function of the hypothalamic-pituitary axis, ovariectomized cynomolgus monkeys were treated with a bolus of estradiol benzoate (E2beta) (To hours) and an i.c.v. injection of 100 microliters of saline solution at T-6 (group I: four monkeys) or at T+26 hours (group II: three monkeys). The lack of disturbance of the normal biphasic luteinizing hormone response to estrogen and low prolactin (PRL) concentrations allow us to conclude that this microinjection procedure did not disrupt normal endocrine events.