Microheterogeneity and Microviscosity of F127 Micelle: The Counter Effects of Urea and Temperature

Abstract

F127 is the most widely studied triblock copolymer and due to the presence of very long polypropylene oxide (PPO) and polyethylene oxide (PEO) groups, F127 micelle has different microenvironments clearly separated into core, corona, and peripheral regions. Urea has been known to have adverse effects on the micellar properties and causes demicellization and solvation; on the other hand, rise in temperature causes micellization and solvent evacuation from the core and corona regions. In the present study, we have investigated the microheterogeneity of the core, corona, and peripheral regions of the F127 micelle using red edge excitation shift (REES) at different temperatures and urea concentrations and correlated the effect of both on the micellar system. It was found that the temperature counteracts the effect of urea and also that the counteraction is more prominent in the core region with respect to corona, and the peripheral region is least affected. Also, the core and corona regions are very much heterogeneous, while the peripheral region is more of a homogeneous nature. Using time-resolved fluorescence anisotropy, we found that the microviscosity within the micelles vary in the order of core > corona > peripheral region, and urea has a general tendency to reduce the microviscosity, especially for core and corona regions. On the other hand, rise in temperature initially increases and then decreases the microviscosity throughout, and at elevated temperatures the effect of urea is being dominated by the effect of temperature, thereby establishing the counter effects of temperature and urea on the F127 micellar system.