Abstract

Decidualization is characterized by the differentiation of endometrial stromal cells (eSCs), which is critical for embryo implantation and maintenance of pregnancy. In the present study, we investigated the possible effect of simulated microgravity (SM) on the process of proliferation and in vitro decidualization using primary human eSCs. Exposure to SM for 36 h decreased the proliferation and migration of eSCs significantly, without inducing cell death and changes in cell cycle progression. The phosphorylation of Akt decreased under SM conditions in human eSCs, accompanied by a simultaneous decrease in the level of matrix metalloproteinase (MMP)-2 and FOXO3a. Treatment with Akti, an Akt inhibitor, decreased MMP-2 expression, but not FOXO3a expression. The decreased level of FOXO3a under SM conditions impeded autophagic flux by reducing the levels of autophagy-related genes. In addition, pre-exposure of eSCs to SM significantly inhibited 8-Br-cAMP induced decidualization, whereas restoration of the growth status under SM conditions by removing 8-Br-cAMP remained unchanged. Treatment of human eSCs with SC-79, an Akt activator, restored the reduced migration of eSCs and decidualization under SM conditions. In conclusion, exposure to SM inhibited decidualization in eSCs by decreasing proliferation and migration through Akt/MMP and FOXO3a/autophagic flux.

Highlights

  • Human space exploration has been growing in recent years

  • We first examined whether a reduced gravitational force affects the growth rate of primary human endometrial stromal cells (eSCs) using a clinostat, a device that is widely used for generating simulated microgravity (SM)

  • Human eSCs grew at a significantly slower rate under SM conditions (30 and 17% reduction was seen at 24 and 36 h, respectively) compared to those grown under terrestrial gravity (Fig. 1A)

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Summary

Introduction

Human space exploration has been growing in recent years. This has led researchers to investigate the effect of harsh environmental conditions, including extreme temperature, ionizing radiation, and altered gravity. Component of cell to cell connection[11], were decreased in eSCs exposed to SM for 36 h (Fig. 2D,E), thereby confirming that exposure to SM leads to slow migration of eSCs. SM reduces Akt activity in human eSCs.

Results
Conclusion
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