Microglia-Derived Exosomes Improve Spinal Cord Functional Recovery after Injury via Inhibiting Oxidative Stress and Promoting the Survival and Function of Endothelia Cells.

Wei Peng,Hongbin Lu,Jianzhong Hu,Zixiang Luo,Liyang Wan,Tingmo Huang,Yong Xie,Yudong Liu,Yuan Xiong

doi:10.1155/2021/1695087

Abstract

Traumatic spinal cord injury (SCI) is a devastating disease of the central nervous system with long-term disability and high mortality worldwide. Revascularization following SCI provides nutritional supports to rebuild and maintain the homeostasis of neuronal networks, and the subsequent promotion of angiogenesis is beneficial for functional recovery. Oxidative stress drastically produced following SCI has been contributed to endothelial dysfunction and the limited endogenous repair of microvasculature. Recently, exosomes, being regarded as potential therapeutic candidates for many kinds of diseases, have attracted great attentions due to its high bioavailability, safety, and stability. Microglia have been reported to exhibit proangiogenic function and guide the forming of vasculature during tissue repair. However, the specific role of microglia-derived exosomes (MG-Exos) played in SCI is still largely unknown. In the present study, we aimed to evaluate whether MG-Exos could protect spinal cord microvascular endothelial cells (SCMECs) against the toxic effects of oxidative stress, thus promote SCMECs' survival and function. We also investigated the protective effects of MG-Exos in the mouse model of SCI to verify their capability. Our results demonstrated that MG-Exo treatment significantly decreased the level of oxidative stress (ROS), as well as did the protein levels of NOX2 when bEnd.3 cells were exposed to H2O2-induced oxidative stress in vitro and in vivo. Functional assays showed that MG-Exos could improve the survival and the ability of tube formation and migration in H2O2-induced bEnd.3 in vitro. Moreover, MG-Exos exhibited the positive effects on vascular regeneration and cell proliferation, as well as functional recovery, in the mouse model of SCI. Mechanically, the keap1/Nrf2/HO-1 signaling pathway was also investigated in order to unveil its molecular mechanism, and the results showed that MG-Exos could increase the protein levels of Nrf2 and HO-1 via inhibiting the keap1; they also triggered the expression of its downstream antioxidative-related genes, such as NQo1, Gclc, Cat, and Gsx1. Our findings indicated that MG-Exos exerted an antioxidant effect and positively modulated vascular regeneration and neurological functional recovery post-SCI by activating keap1/Nrf2/HO-1 signaling.

Highlights

Traumatic spinal cord injury (SCI) is a devastating disease of the central nervous system, which may cause major motor, sensory and autonomic dysfunctions, and bring far-reaching adverse consequences for patients, families, and society [1]
To evaluate movement recovery after SCI, the motor-evoked potentials (MEPs) of hindlimbs were recorded by electromyography conducted at 4 weeks post-SCI as described in our previous research [34]
After anesthesia, the stimulating electrodes were secured onto the surface of the skull corresponding to the motor area of the cerebral cortex, while the recording electrodes were inserted into the tibialis anterior muscle of the contralateral hindlimb

Summary

Introduction

Traumatic spinal cord injury (SCI) is a devastating disease of the central nervous system, which may cause major motor, sensory and autonomic dysfunctions, and bring far-reaching adverse consequences for patients, families, and society [1]. Contusion injury, followed by hypoxia, ischemiareperfusion injury (SCIRI), and microenvironment imbalance, can cause the production and release of large amounts of free oxygen radicals and reactive oxygen species (ROS) [3, 4]. These strong oxidants accumulate in the tissues leading to topical microvasculature disorder and the dysfunction of myelin sheaths of nerve cells [5,6,7,8]. High level of oxidative stress and poor microenvironment limited the endogenous repair of microvasculature and the regeneration of axons following SCI [16, 17]. Quickly balancing the oxidative stress of the spinal cord is an important way to improve angiogenesis, restore blood flow, and protect the function of spinal cord microvasculature and nerve cells

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