Abstract
A combined experimental and numerical analysis technique for velocity profiles with near-wall resolution in liquid microchannel flows is described. The working fluids employed are aqueous solutions with caged fluorescent dyes. A sheet of fluorescent dye is photo-injected by briefly exposing a cross-section of the fluid to ultraviolet light. The transport of the resulting ‘band’ of fluorescent dye is imaged onto a CCD camera using an epi-illumination fluorescent microscope system. The velocity profile is calculated from images acquired and processed after each uncaging event. Results are shown to compare favorably to analytical solutions in both pressure-driven and electroosmotic flow in circular cross-section capillaries. Near-wall resolution is verified through application to electroosmotic flows with thin electrical double layers. The ability of the technique to collect velocimetry data up to 2.5 μm from the wall is demonstrated.
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