Abstract
Miniaturization of different measurement processes and a scaled-down approach open the possibility for rapid measurements with the small amounts of samples and reagents into a compact platform with integrated sensors and different measuring components. In this paper, we report a microfluidic approach for measurements of salivary pH, dissolved O2, and CO2 during chewing xylitol gum. The study was done with the samples of 30 healthy volunteers who were chewing a xylitol gum, and the measurements were performed in the microfluidic (MF) chip with integrated commercial PreSens sensors. Xylitol exhibited a significant effect on the pH of saliva in terms of its initial drop, which was the most significant between the 5th and 10th minutes. The effect of xylitol on the amount of oxygen and carbon dioxide in saliva cannot be confirmed. The employed microfluidic platform was shown to be applicable and effective in the analysis of salivary biomarkers relevant to caries development.
Highlights
Microfluidics is the most advanced field of modern science and technology, due to a wide range of applications in different fields, such as medicine, biology, chemistry, or environmental protection [1,2,3]
Paired T-test was employed for intergroup analysis and revealed that the drop of salivary pH in the period between the first and fifth minutes from the beginning of the experiment was statistically significant (p = 0.025, Table 4)
This study presents the possibility of using a microfluidic platform in the analysis of salivary biomarkers of importance for the development of caries after the use of xylitol
Summary
Microfluidics is the most advanced field of modern science and technology, due to a wide range of applications in different fields, such as medicine, biology, chemistry, or environmental protection [1,2,3]. The use of salivary samples for both oral and general health biomarkers’ detection gains increasing attention from scientists and clinicians since saliva presents an accessible and sophisticated diagnostic fluid with a great potential for integration in various microfluidic setups [7]. In recent years, these systems have even exploited microelectronics, biotechnologies, and integration into smartphones to provide portable and miniaturized analytical devices. Of particular importance are microfluidic systems that allow simultaneous detection of multiple biomarkers in real time
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