Microfluidic on-chip production of microgels using combined geometries

Hamed Shieh,Maryam Saadatmand,Dariush Bastani,Mahnaz Eskandari

doi:10.1038/s41598-021-81214-7

Hamed Shieh, Maryam Saadatmand + Show 2 more

Open Access

https://doi.org/10.1038/s41598-021-81214-7

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Abstract

Microfluidic on-chip production of microgels using external gelation can serve numerous applications that involve encapsulation of sensitive cargos. Nevertheless, on-chip production of microgels in microfluidic devices can be challenging due to problems induced by the rapid increase in precursor solution viscosity like clogging. Here, a novel design incorporating a step, which includes a sudden increase in cross-sectional area, before a flow-focusing nozzle was proposed for microfluidic droplet generators. Besides, a shielding oil phase was utilized to avoid the occurrence of emulsification and gelation stages simultaneously. The step which was located before the flow-focusing nozzle facilitated the full shielding of the dispersed phase due to 3-dimensional fluid flow in this geometry. The results showed that the microfluidic device was capable of generating highly monodispersed spherical droplets (CV < 2% for step and CV < 5% for flow-focusing nozzle) with an average diameter in the range of 90–190 μm, both in step and flow-focusing nozzle. Moreover, it was proved that the device could adequately create a shelter for the dispersed phase regardless of the droplet formation locus. The ability of this microfluidic device in the production of microgels was validated by creating alginate microgels (with an average diameter of ~ 100 μm) through an external gelation process with on-chip calcium chloride emulsion in mineral oil.

Highlights

Microfluidic on-chip production of microgels using external gelation can serve numerous applications that involve encapsulation of sensitive cargos
We developed a microfluidic device for the production of highly monodispersed spherical microgels
A novel design that incorporates a step before a flow-focusing nozzle was used for droplet formation

Summary

Introduction

Microfluidic on-chip production of microgels using external gelation can serve numerous applications that involve encapsulation of sensitive cargos. On-chip production of microgels in microfluidic devices can be challenging due to problems induced by the rapid increase in precursor solution viscosity like clogging. It was proved that the device could adequately create a shelter for the dispersed phase regardless of the droplet formation locus The ability of this microfluidic device in the production of microgels was validated by creating alginate microgels (with an average diameter of ~ 100 μm) through an external gelation process with on-chip calcium chloride emulsion in mineral oil. The microchannel and nozzles may be clogged In these systems, to control the dispersity and morphology of generated microgels, there should be a time delay between the emulsification and gelation p rocesses[7]. After droplet formation, the crosslinker diffused into droplets and cross-linked the PEG-4MAL macromer into the hydrogel n etwork[13,14]

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