Abstract
Sensitive and specific DNA biomarker detection is critical for accurately diagnosing a broad range of clinical conditions. However, the incorporation of such biosensing structures in integrated microfluidic devices is often complicated by the need for an additional labelling step to be implemented on the device. In this review we focused on presenting recent advances in label-free DNA biosensor technology, with a particular focus on microfluidic integrated devices. The key biosensing approaches miniaturized in flow-cell structures were presented, followed by more sophisticated microfluidic devices and higher integration examples in the literature. The option of full DNA sequencing on microfluidic chips via nanopore technology was highlighted, along with current developments in the commercialization of microfluidic, label-free DNA detection devices.
Highlights
IntroductionThe rapid technological progress of the past couple of decades in the fields of biosensors and microfluidics [1], has allowed the originally envisioned democratization of healthcare of the early
The rapid technological progress of the past couple of decades in the fields of biosensors and microfluidics [1], has allowed the originally envisioned democratization of healthcare of the early1990s [2] to be closer to realization than ever
We reviewed recent technological progress in the field of microfluidic-assisted label-free DNA biosensing devices
Summary
The rapid technological progress of the past couple of decades in the fields of biosensors and microfluidics [1], has allowed the originally envisioned democratization of healthcare of the early. A broad range of different biosensing concepts and microfluidic structures currently allows the qualitative or quantitative detection of various biomarkers of clinical interest. With the ultimate goal being affordable, easy to use and small footprint DNA diagnostic microsystems, microfluidic structure integration for sample pre-processing and automated reagent handling are some of the critical aspects being investigated currently [5]. In this review our goal is to present recent developments in label-free DNA biosensors, with a particular focus on integration with microfluidic structures. Chemosensors 2018, 6, 43 molecule on the target DNA sequence; techniques involving the use of reagents diluted in solutions for measurement purposes, are still to be label-free. DNA sequence; techniques involving theconsidered use of reagents diluted in solutions for measurement purposes, are still considered to be label-free
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