Abstract
Dry eye is a problem in tearing quality and/or quantity and it afflicts millions of persons worldwide. An autologous serum eye-drop is a good candidate for dry eye treatment; however, the eye-drop preparation procedures take a long time and are relatively troublesome. Here we use spiral microchannels to demonstrate a strategy for the preparation of autologous serum eye-drops, which provide benefits for all dry eye patients; 100% and 90% removal efficiencies are achieved for 10 μm microbeads and whole human blood cells, respectively. Since our strategy allows researchers to integrate other functional microchannels into one device, such a microfluidic device will be able to offer a new one-step preparation system for autologous serum eye-drops.
Highlights
2 of 7 2 of 7 eye-drops can be stored for up to three months at −80 °C
Dry eye is a problem in tearing quality and/or quantity and it afflicts millions of persons worldwide
An autologous serum eye-drop is a good candidate for dry eye treatment; the eye-drop preparation procedures take a long time and are relatively troublesome
Summary
2 of 7 2 of 7 eye-drops can be stored for up to three months at −80 °C. The autologous serum eye-drops are pcarnepbaeresdtoaresdfoflolrowups: tfoirtsht,reaepmatoienntht’ss abtloo8d0 isCc.oTllheectaeudtoinloagohuespsaerrinu-munemyeo-ddirfioepds balroeopdrecpoallreecdtioans tfuolbleo;wsse:cofinrsdtl,ya, pthaeticeonltl’escbtelodobdloisodcoilsleccetnetdriifnugaehdeapta3ri0n0-0urnpmmodfoifir e1d0 bmloino;dthciorldlelyct,itohnetsuubpee;rsneactoanndt liys, fthiletecroedlletchtreodubglhooad0.i4s5c-eμnmtr-ipfuogree-dsiazte3f0il0t0err;pamndfofirn1a0llmy, itnh;ethfiilrtdelrye,dthseersuumpeirsndaitlaunttedis tfioltreeraecdhtahrtoaruggeht cao0n.c4e5n-μtrmat-ipoonrue-ssiinzgesfialltienre;. aHnodwfienvaelrl,yt,hteheprfielptearreadtiosneriusmreliastidvielluytetrdoutoblreesaocmh eaatnadrgteatkceosnacleonntgrattiimone duusiengtostahleincee.nHtriofwugeavteior,nt,hfeiltpraretipoanr,aatniodndiisluretiloantivsteelypst.roublesome and takes a long time due to the centrHifuergeatwioen,dfielmtroantisotnra, taenddadislturtaitoengystefoprs.the preparation of autologous serum eye-drops using a microHfleuriedwice tdeecmhnoinquster.ateMdicarosftrluaitdegicys fohrastheshporwepnaragtrieoant opf raoumtoilsoegofuors sseirgunmificeaynet-ldyroipmspursoivnignga dmiiacgronfloustiidcisc, taeschwneilqluaes. bMioiclorogflicuaildaicnsdhmasesdhiocawlnregsreeaartcphrsotmudisieesfo[r5]s.igMniicfircoafnlutliydiimcsphraosvibnegendivagarnioosutsiclys, uassewdelfloars pbaioslsoivgeicablloanodd mceeldlsicsaelpreasreaatirocnh satpupdrieosa[c5h]e. sM[i6c]r,osfluucihdicass hhaysdbreoednyvnaarmioiucslsyepuaseradtifoonr p[a7s–s1i7v]e, sbelodoimd ecneltlastisoenp-abraasteiodnsaeppparroaaticohnes[1[68]–,2s1u]c,haansdhfyildtrroatdioynn-abmasicedsepseapraatriaotnio[n7–[1272]–, 3s3e]d.imCoennstaidtieornin-bgastehde dseepsairreatifoonr [h1i8g–h21t]h, raonudgfihlptruattioann-dbatsheed nseepedaraftoirona[2f2u–r3th3e].rCdoinlusitdioenrinpgrothceesds,eswiree ffoarbhriigcahtethdroaugsphpiruatl manidcrtohcehnaneendelfo(rFaigfuurrethe1ra)ditluotiroenalpizroeceisnse,rwtiaelfambriigcraatteidona, sponireal omfictrhoechhayndnreol d(Fyingaumreic1as)etpoarreaatliiozne tinecehrtniaiqlumesig[r3a4t]io. nIn, ocnuervoifntghemhicyrdorcohdaynnnaemls,icpsaerptiaclreastioexnpteercihennciqeuaesco[3m4b].inInatciounrvoinf ginmeritciraol clhifatnfnoerclse, panadrtiDcleeasnexdpraergiefnorcceea; icnoemrtbiainl alitfitofnorocfeinaectrstitaol lfioftcufosrmceicarnodbeDaedasnadt raangefqourcieli;birniuermtiaplolisfittifoonrcbeeatwctseetno tfhoceucshmanicnreolbweaadlls aant danceenqtueirlliibnreiu[m35,p3o6]s,itaionndbDetewaneednrtahgefcohracennaecltswtaolleanntdracinenmteirclrionbee[a3d5s,36a]s, atwndo cDoeuanntedrr-argotfaotricnegavcotsrttioceesnwtraitihn fmloiwcrodbiereacdtsedastotwwoarcdouthneteor-urtoetratbienngdvaotrttihceesmwiditlhinfleoowf tdhierecchteadnntoewl aanrdd itnhwe aorudtseratbtehnedcahtatnhneelmeiddgliense[3o7f,3th8]e. Microfluidic Autologous Serum Eye-Drops Preparation as a Potential Dry Eye Treatment We use spiral microchannels to demonstrate a strategy for the preparation of autologous serum eye-drops, which provide benefits for all dry eye patients; 100% and 90% removal efficiencies are achieved for 10 μm microbeads and whole human blood cells, respectively.
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