Microflow injection chemiluminescence system with spiral microchannel for the determination of cisplatin in human serum

Abstract

A new microflow injection chemiluminescence (μFI-CL) system was described for the determination of cisplatin in human serum. By using the microchip with double spiral channel configuration, the sensitivity was greatly enhanced due to more efficient mixing of the analyte and reagent solutions. Experimental results revealed that common ions in human serum, such as Mn 2+, Co 2+, Fe 3+, Cu 2+, Zn 2+, Ni 2+, Na +, K +, Ca 2+, Cl −, NO 3 −, Ac −, CO 3 2−, PO 4 3−, SO 4 2− did not cause interference with the detection of Pt(II) by using 1,10-phenanthroline as the masking agent. Under the optimized conditions, a linear calibration curve ( R 2 = 0.998) over the range 2.0 × 10 −8 to 2.0 × 10 −6 mol L −1 was obtained with the detection limit of 1.24 × 10 −9 mol L −1. The relative standard deviation was found to be 3.46% ( n = 12) for 2.0 × 10 −7 mol L −1. The sample consumption was only 2 μL with the sample throughput of 72 h −1. It had been used for trace platinum determination in cisplatin injection and human serum samples after the dosage of cisplatin. The recovery varied from 97.6 to 103.9%. The results proved that the proposed μFI-CL system had the advantages of high sensitivity and precision, low sample and reagents consumption, and high analytical throughput.