Abstract
The purpose of current work was isolation of halophilic bacteria from saline lands in some regions of the Krasnodar Territory, after mycological analysis, there followed the study and characterization of microorganisms with further creation on effective biocomplex basis, contributing to restoration of arid and saline irrigated areas. Soil samples were taken from saline lands of the Krasnodar Territory, the salt composition of which was studied in the work process. There were determined total titer of microorganisms and the titer of nitrogen-fixing bacteria on the Corresponding selective media in the samples. Twenty-five pure bacterial cultures were isolated from the samples and its morphophysiological and biochemical properties, as well as its nitrogenfixing activity, were studied. As a result, seven cultures with relatively high nitrogen-fixing activity were selected, as well as resistance to various environmental factors, particularly, to high concentrations of NaCl, рН alkaline values and to sharp temperature drops. After appropriate phylogenetic analysis, selected crops may become a potential target for creating a complex biological fertilizer, contributing to the restoration of dry and saline lands and increase their fertility.
Highlights
Land degradation under the influence of salts is a serious threat to Russian agriculture
The main causes of the problem are intensification of production, reduction of areas with high natural agroresource potential, growth in demand for agricultural products, quality deterioration and irrigation water quantity reduction, lack of proper attention to the methods for producing agricultural products: crop rotation failure, irrigation regimes violation, failure to comply with all regulated autumn-spring field work [1, 2]
Reclamation of saline arable land is very relevant for Russia as a whole and for the Kuban in particular, since at present the damage caused by salinization is increasing
Summary
Land degradation under the influence of salts is a serious threat to Russian agriculture. The main causes of the problem are intensification of production, reduction of areas with high natural agroresource potential, growth in demand for agricultural products, quality deterioration and irrigation water quantity reduction, lack of proper attention to the methods for producing agricultural products: crop rotation failure, irrigation regimes violation, failure to comply with all regulated autumn-spring field work [1, 2]. It is important to understand the processes occurring in saline soils, These problems can be solved with biofertilizers, as some microbes respond to low osmotic potential, accumulating osmolytes to retain water, while sensitive microbes die [7, 8]. Considering the above, the main goal of current work is: study of soil biota (fungi, bacteria), isolation of (N) fixing microorganisms from saline lands of the Krasnodar Territory, study of its characteristics, followed by the use of salt tolerant microorganisms as bio-fertilizers in saline soil
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