Microflora in Rats Metagenomic Analysis of Intestinal Microflora in Rats Treated with Ellagic Acid and Sinapic Acid Using 16 S rDNA Gene Region

Abstract

Abstract: Background: The correct functioning of the intestinal mechanism is essential for human health. In particular, the micro ecosystem of the gut has a vital role in the development of the host and the continuity of metabolism. For this reason, many studies have been conducted on the intestinal microbiota. Today, the use of phenolic compounds in the production of complementary and alternative medicines is quite common. Purpose: In our study, changes in intestinal microflora at the species level were evaluated in Wistar Albino rats treated with Ellagic Acid and Sinapic Acid. Materials and Methods: The first group was formed as the control group. Ellagic Acid (EA) was given to the second group of rats (50 mg/kg/d). The third group of rats was given Sinapic Acid (SA) (20 mg/kg/d). The fourth group of rats was given Ellagic Acid EA (50 mg/kg/d) and Sinapic Acid SA (20 mg/kg/d). At the end of the study, intestinal tissues were taken under appropriate sterile conditions. The V3-V4 points located in the 16 S rDNA gene regions of the bacteria in the microflora were replicated by the specific primers developed in accordance with these regions. Results and Conclusion: The data obtained from of sequencing analyzes were evaluated comparatively for four groups. As a result, it was determined that Ellagic Acid and Sinapic Acid have a positive effect on probiotic microorganisms (Lactobacillus) in the intestine. It was also determined that while it caused a decrease in the rate of some pathogenic microorganisms such as Streptococcus, it caused an increase in the number of some pathogens. Keywords: Gut microbiota, Metagenome, Ellagic acid, Sinapic acid, 16 S rDNA.