Abstract
A novel microfiltration batch culture process, suitable for scale-up, has been designed, instrumented, and studied with a representative recombinant-DNA receptor strain of Escherichia coli. In this process nutrient medium is fed continuously into an aerated and stirred fermentor, and metabolic products are withdrawn continuously at the same rate by crossflow microfiltration in an external recycle loop, so that product inhibition of cell growth is relieved. The maximum density of cells is increased fivefold more than in the control process, and is limited by acetic and other organic acids accumulating from the aerobic fermentation of excess glucose.
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