Abstract
Summary Bundles of microfilaments have been obtained from a fraction of homogenised amoebae cells after the addition of EDTA. These bundles are made up of many single microfilaments 30–40 A in diameter. An attempt has been made to obtain the protein subunit of these microfilaments. In the detergent Sarkosyl, a subunit of sedimentation constant 2.0×10−13 and mol. wt approx. 42 500 has been found. The amino acid composition of this protein is similar to that of muscle actin, plasmodium actin and microtubule subunit protein. On removing the Sarkosyl, the protein reaggregates into bundles of microfilaments like those originally formed with EDTA.
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