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Abstract
Micropore arrays have attracted a substantial amount of attention due to their strong capability to separate specific cell types, such as rare tumor cells, from a heterogeneous sample and to perform cell assays on a single cell level. Micropore array filtration has been widely used in rare cell type separation because of its potential for a high sample throughput, which is a key parameter for practical clinical applications. However, most of the present micropore arrays suffer from a low throughput, resulting from a low porosity. Therefore, a robust microfabrication process for high-porosity micropore arrays is urgently demanded. This study investigated four microfabrication processes for micropore array preparation in parallel. The results revealed that the Parylene-C molding technique with a silicon micropillar array as the template is the optimized strategy for the robust preparation of a large-area and high-porosity micropore array, along with a high size controllability. The Parylene-C molding technique is compatible with the traditional micromechanical system (MEMS) process and ready for scale-up manufacture. The prepared Parylene-C micropore array is promising for various applications, such as rare tumor cell separation and cell assays in liquid biopsy for cancer precision medicine.
Highlights
Micropore arrays have attracted lots of attention due to their capability in cell operations at the single cell level, especially rare tumor cell separation and cell assays from a large volume of clinical samples in the liquid biopsy
The competitiveness of micropore array-based filtration among the developed techniques for liquid biopsy is its promising potential to realize a high throughput at mL/min, along with a high recovery rate [1,2,3,4]
Si micropore arrays with an area of 0.64 cm2 and porosity of 10% were produced via deep reactive iron etching (DRIE) and KOH etching approaches by Wit et al [9]
Summary
Micropore arrays have attracted lots of attention due to their capability in cell operations at the single cell level, especially rare tumor cell separation and cell assays from a large volume of clinical samples in the liquid biopsy. For the track-etched micropore array, it is easy to realize a large area; the size and geometry are uncontrollable (with fusion of two or more pores), and the porosity is very low (less than 1%), resulting from the random placement of pores with a relatively low density. Fan et al utilized a sandwich molding technique (modified soft lithography) for the preparation of a polydimethylsiloxane (PDMS) micropore-arrayed membrane from a microfabricated silicon micropillar-arrayed master.
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