Abstract
According to descriptions, the Siberian species E. peschkovae and E. confusus differ in the presence or absence of trichomes on lemma and rachilla surfaces only. Two methods were used for study microevolutionary relationships between these species: hybridization of biotypes from different locations and analysis of nucleotide sequences of the low-copy nuclear gene GBSS1. Created and grown hybrids in combinations AMU-8804 × BER-0807 and AMU-8804 × BUK-1109 showed complete seed sterility in two field vegetations. Taking into account wide species areas, 3 hybrids were created between Magadan biotypes from a common habitat in the combination E. confusus MOL1887 × E. peschkovae MOL-1882. The plants showed seed fertility (SF) of 0–3 %. This result does not obscure the possibility to assess SF in the F2 generation and the character of inheritance of diagnostic traits. A comparative study of the GBSS1 gene sequences in accessions was carried out in comparison with clones of reference species. Differentiation of clones by the St2 subgenome in E. confusus, E. peschkovae, E. sibiricus and E. caninus did not reveal a clear relationships between the species. Meanwhile, a certain species specificity for the H1 subgenome was noted, confirming the existence of microevolutionary isolation of these species.
Highlights
Elymus sibiricus L. and E. confusus (Roshev.) Tzvel. are spreaded everywhere in southern regions of Siberia [1]
In the Russian Far East E. confusus area is shifted to the North, where it totally replaces E. sibiricus [2]
Created and analyzed hybrids of East-Sajan biotypes in combination E. sibiricus BUR0569 × E. confusus BUM-0505 in generation F1 showed mostly opened anthers, values of seed fertility (SF) accounted for 2–7 seeds per spike
Summary
Elymus sibiricus L. and E. confusus (Roshev.) Tzvel. are spreaded everywhere in southern regions of Siberia [1]. In the Russian Far East E. confusus area is shifted to the North, where it totally replaces E. sibiricus [2] Both species are characterized by glabrous lemmas, but E. sibiricus rarely have shortly pilous lemmas. Among 20 plants of the F2 generation, grown on the opened plot, 17 corresponded to E. sibiricus on the trait of doubled spikelets on spike ledge, 3 plants carried single spikelets on all spikes. These results gave reason to refer E. sibiricus and E. confusus to a unified recombination gene pool with reproductive compatibility at the α2 level at least within the Southern Siberia area
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