Abstract
The coacervation technique is an efficient encapsulation process in which macromolecules with opposite surface charges interact, resulting in polymeric complexes. Hydroethanolic solvent (52%) was selected to obtain bioactive compounds from red beet using the mixture experimental design. Red beet extract was encapsulated in a complex of chitosan and negatively charged Persian gum. The optimum condition for coacervate formation was received at the chitosan to Persian gum ratio of 18:82 at pH 5.3. The coacervation yield of the two polysaccharides was 80%, and the zeta potential of dispersion was close to zero. Complex formation between two biopolymers was confirmed through FTIR. Micro-sized coacervates showed 90% encapsulation efficiency for the betalain extract to coacervate dispersion ratio of 14.3%. The betalain-loaded chitosan/Persian gum complex presented superior thermal stability compared with pure polysaccharides. The obtained complex can be applied to improve the stability of betalains and control their release in food and pharmaceutical applications. Novelty Impact Statement Equal mixture of water:ethanol resulted in maximum betalain extraction. Strong electrostatic complexation was approved between Persian gum and chitosan. Persian gum/chitosan coacervate demonstrated high loading capacity for betalains.
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