Microencapsulation of Lemongrass Leaves Effect on Reactive Oxygen Species (ROS) Fibroblasts

Abstract

Tissue damage during the inflammatory phase produces neutrophils and macrophages, which leads to excessive levels of reactive oxygen species (ROS). Excessive ROS level can cause progressive oxidative damage, thereby inhibiting wound healing. Cymbopogon citratus (lemongrass) leaves have active antioxidant compounds encapsulated with chitosan polymer, which improve the stability and effectiveness of active compounds in wound healing. This study examines the reduction of ROS production in fibroblasts under oxidative stress by encapsulating lemongrass (EChLgm). The study was divided into ten groups, untreated group, ascorbic acid, hydrogen peroxide, lemongrass extract and chitosan as controls, and five EChLmg concentrations of 100 ppm, 50 ppm, 25 ppm, 12.5 ppm and 6.5 ppm. The higher the EChLmg concentration, the more effective in reducing the ROS production on fibroblasts. The production of ROS on fibroblasts formed without the induction of H 2 O 2 which was shown at a percentage of 50.62%. Ascorbic acid has the best ability to reduce ROS production with the intensity of 43.67%, while the stressor group produced the highest ROS with the intensity of 53.68%. The result showed that EChLmg group reduced ROS production on fibroblasts, with the best concentration of EChLmg at 100 ppm, with the intensity of ROS of 47.62%.