Abstract
Objective: WJMSC is a stem cell-derived from Wharton's Jelly the umbilical cord of pregnant women which has the ability to differentiate into other cells that are osetogenic, myogenic, neurogenic, and hematopoietic. Stem cell microencapsulation is a cell coating technique that is expected to act as a delivery vehicle. This study aims to make stem cell microencapsulation using various types of natural polymers.
 Methods: WJMSC (Wharton's Jelly Mesenchymal Stem Cell) was cultured with supplemented Modified Eagle Medium (MEM) Alpha in incubator with 37 °C and 5% of CO2. In this study, we investigated various of natural polymers (chitosan, glucomannan, inulin, fucoidan and amylopectin) in WJMSC microencapsulation. Viability cell of WJMSC microencapsulate was measured using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay, and the condition medium (CM) was determined the measure EGF, IL-6, VEGF with ELISA.
 Results: WJMSC microencapsulation using chitosan, oxidized glucomannan, inulin, fucoidan and oxidized amylopectin showed viability cell up to 100%. The EGF, IL-6, VEGF levels were increased in all tested polymers compared to negative control.
 Conclusion: Tested polymers (chitosan, oxidized glucomannan, inulin, fucoidan, oxidized amylopectin) were not toxic to WJMSC and cell microencapsulation was successfully carried out.
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