Abstract
Karyotypes of two cryptic species of parasitoid Hymenoptera with n = 5 and 6 belonging to the Lariophagus distinguendus (Förster, 1841) complex, which includes cosmopolitan parasitoids of coleopteran stored-product pests, were studied using glass-needle based microdissection, reverse and cross-species fluorescence in situ hybridisation (FISH). This experiment strongly indicates that the largest metacentric chromosome in the karyotype with n = 5 originated from a particular fusion between the only acrocentric and a smaller metacentric chromosome of the set with n = 6, therefore confirming our previous hypothesis based on the karyotypic analysis using chromosome morphometrics. This study represents the first successful application of both microdissection and whole chromosome painting for the reconstruction of karyotypic rearrangements in closely related species of parasitoids, as well as in the order Hymenoptera in general.
Highlights
Parasitoid Hymenoptera are one of the most species-rich, taxonomically complicated and economically important groups of insects [1]
Two strains of L. distinguendus, PFO-D and RAV-D, which were respectively collected in Pforzheim and Ravensburg (Baden-Wurttemberg, Germany) and kept as lab stocks at the Institute for Zoology, University of Hohenheim, Stuttgart, Germany [23,24,25] were used in the present study
The experiments with the chromosome set of L. distinguendus (n = 6) demonstrated that LD6-A probe hybridised with the only A pair (Fig 2B), while LD5-1 probe marked these chromosomes as well as another relatively small chromosome pair (Fig 2C)
Summary
Parasitoid Hymenoptera are one of the most species-rich, taxonomically complicated and economically important groups of insects [1]. Its world fauna contains more than 80 thousand described species [2]. Karyotypes of only about 500 members of this group have been examined up to now [3]. Transformations of parasitoid karyotypes are poorly studied, mainly due to the lack of corresponding research by appropriate methods [4]. [5]) identified certain putative karyotypic rearrangements distinguishing closely related parasitoid species. A few recent studies involving chromosome morphometrics These results were rarely confirmed by any molecular technique [5]
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