Microdialysis Sampling of Renal Interstitial Fluid in Acute Studies

Wael Alanazi,Keith E Jackson,Selim Fakhruddin

doi:10.5539/ijb.v8n1p69

Abstract

<p class="1Body">The microdialysis technique has been applied extensively during the last three decades where most of the research has focused primarily on the site of action. The microdialysis probe was developed for collection of interstitial fluid as well as delivering drugs to the target site. In comparison with other methods, the microdialysis technique has delivered more accurate results with minimal invasive procedures. Initially, the microdialysis technique was designed to quantitate dopamine levels in cerebral tissues, which facilitated the applications of this technology as a sampling tool. Presently the microdialysis technique has been applied to various organs such as the heart, kidney, and liver. In the current report, we describe the principle of the microdialysis technique and its application in <em>in-vivo</em> studies. Specifically, the present review provides the adaptation of this method in renal acute studies. However, the rationale for this report is to demonstrate that the microdialysis technique can be used to collect renal interstitial fluid in real time with no effect on the hemodynamic function.</p>

Highlights

The importance of interstitial fluid (ISF) sampling is its similarity to plasma in that it is the primary component of the extracellular fluid, and comprised of interstitial water, proteins, and other solutes
We describe the principle of the microdialysis technique and its application in in-vivo studies
Advances over the years in microdialysis probe construction provide an advantage over the push-pull technique, where microdialysis probes are less invasive, and contain semipermeable membranes, which allow for collection of a wide range of proteins

Summary

Introduction

The importance of ISF sampling is its similarity to plasma in that it is the primary component of the extracellular fluid, and comprised of interstitial water, proteins, and other solutes. The disadvantages of push-pull techniques were overcome by Ungerstedt and Pycock They employed a microdialysis probe that had a hollow fiber for continuous monitoring of dopamine levels in cerebral ISF (Ungerstedt, & Pycock, 1974). During the last thirty years, numerous studies have shown the importance of the microdialysis technique in collecting cerebral ISF. Advances over the years in microdialysis probe construction provide an advantage over the push-pull technique, where microdialysis probes are less invasive, and contain semipermeable membranes, which allow for collection of a wide range of proteins. Microdialysis produces a more accurate assessment of real-time solute changes in comparison to other sampling techniques

Principle of Microdialysis

Microdialysis Applications in in-vivo Study

Adaptation of Microdialysis Technique in Acute Renal Studies

Advantages of using Microdialysis Technique in Acute Studies

Conclusion