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Abstract
Utilising microdialysis to measure the changes of glucose, lactate, pyruvate and glutamate levels in the spinal cord after cross-clamping of the thoracic aorta in an established porcine model to monitor the degree of ischaemia. Experimental study with a porcine model. University Hospital, Trondheim. Six pigs. Lactate, pyruvate and glutamate concentrations in the microdialysis perfusate from the spinal cord. A significant increase of the lactate-pyruvate ratio during the last 30 min of the 1 h clamping period, with a maximum increase of 169% from the basal value the last 10 min before declamping. No evident change in this ratio between the clamping and the reperfusion period. No significant change in glutamate levels during clamping or reperfusion period. Microdialysis reflects the ischaemic state of the spinal cord during cross-clamping of the thoracic aorta in pigs, and is well suited to study each phenomena.
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