Microdialysis in the posterior hypothalamus: Sodium chloride affects norepinephrine release, mean arterial pressure, heart rate and behavior in awake rats

Abstract

A microdialysis probe, implanted in the posterior hypothalamus (PH) was used to examine changes of extracellular norepinephrine (NE) in freely moving rats from which mean arterial pressure (mAP) and heart rate (HR) were continuously monitored. Artificial cerebrospinal fluid (aCSF) was pumped through the probe and 10-μl dialysate samples were collected at 5-minute intervals and analyzed for NE by radioenzymatic assay. High sodium chloride (NaCl)-aCSF solution elicited pressor and tachycardiac responses and locomotor activity coupled with significant increases in levels of dialysate NE. The latter and the associated cardiovascular effects were significantly attenuated by perfusate lidocaine (0.5%). When alpha-adrenergic receptors in PH were blocked by phenoxybenzamine (0.165 M), high NaCl-aCSF released NE but the associated cardiovascular effects were attenuated. In addition, intravenous ganglionic blockade with hexamethonium (25 mg/kg) also attenuated cardiovascular responses during the high NaCl-aCSF perfusion of PH. These results indicate that PH is one of the important areas for central actions of NaCl and that the cardiovascular and locomotor responses produced by central NaCl, in part, depend on neuroadrenergic activity in PH.