Microdetermination of Proteins with the 1,10-Phenanthroline–H2O2–Cetyltrimethylammonium Bromide–Cu(II) Chemiluminescence System

Abstract

Proteins can enhance the chemiluminescence (CL) intensity of the 1,10-phenanthroline–H2O2–cetyltrimethylammonium bromide (CTMAB)–Cu(II) system because unsaturated complexes of Cu(II) with protein have a much stronger catalytic effect on the CL reaction than does Cu(II). On this basis, a new flow injection analysis method for detection of some proteins was established. The method gives linear responses over two orders of magnitude and detection limits at the 0.02–0.05 μg ml−1level for bovine serum albumin, human serum albumin, γ-globulin, and egg albumin. The method was used for determination of proteins in human serum with satisfactory results.