Abstract
This work focuses on detection of microcystin-LR (MC-LR) in direct immunoassay with specific monoclonal antibody (MC10E7) using optical technique of total internal reflection ellipsometry (TIRE) as well as direct UV–vis spectra measurements. The concentration of MC-LR toxin produced naturally by bacterial algae Microcystis aeruginosa was evaluated using calibration data obtained for aqueous solutions spiked with known concentrations of commercially available MC-LR. The detection limit for MC-LR between 0.1 and 1ng/ml was achieved using TIRE detection. The role of environmental factors (temperature, light, nutrient contents, salinity) on the efficiency of production of MC-LR by M. aeruginosa was studied.Purification of solutions contaminated with MC-LR (both commercial and naturally produced) was achieved using MnCO3 microparticles coated with polyelecrolytes and functionalized with MC10E7 antibodies as an absorbent for MC-LR. Also, the concentration of MC-LR in solutions was reduced as a result of direct electrolysis.
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