Abstract
A microtechnique for marmoset lymphocyte cultures is described that allows a 10-fold reduction of cell numbers and the amount of culture medium and radioisotopes. Optimal culture conditions for the response of Saguinus fuscicollis, Saguinus oedipus, and Callithrix jacchus peripheral blood lymphocytes to a variety of mitogens were established. The usefulness of marmoset lymphocyte microcultures for the evaluation of alterations of immunocompetence associated with experimental viral disease and its possible application for immunogenetic studies are discussed.
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