Abstract
Microcontact printing (µCP) is an easy and efficient way of producing patterns of self-assembled monolayers (SAM) containing different functional groups. We have developed a simple and convenient µCP-based technique for the modification of a mica substrate with 3-aminopropyltriethoxysilane (APTES) and the micropatterning of proteins (chicken IgG and rabbit IgG) on the modified mica surface. Our approach provides a quick and easy way to produce protein patterns on solid surfaces. The printed immunoglobulin patterns were detected by exposing the substrate to solutions containing fluorescently labeled complementary anti-IgGs, and the formed immunoassays were studied using fluorescence microscopy. Copyright © 2005 John Wiley & Sons, Ltd.
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