Abstract
Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 × 106–15 × 106 CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of Salmonella paratyphi was achieved using these SPR sensor with a detection limit of 1.4 × 106 CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring Salmonella paratyphi in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies.
Highlights
Salmonella paratyphi (S. paratyphi) is known as one of the major globally distributed pathogenic bacteria and a leading cause of foodborne diseases [1]
S. paratyphi imprinted Surface Plasmon Resonance (SPR) chips were prepared by microcontact imprinting method with the bacterial stamp and monomer mixture covering modified SPR chip surface (Figure 1)
The interactions between the imprinted cavities made for the target bacterial strain and the polymeric film resulted in high affinity of the bacterial cells for imprinted nano-cavities
Summary
Salmonella paratyphi (S. paratyphi) is known as one of the major globally distributed pathogenic bacteria and a leading cause of foodborne diseases [1]. These diseases represent a major threat due to their significantly increased incidence throughout the world [2]. It is important to be able to detect pathogenic microorganisms in food to provide real-time quality results [3]. Rapid detection of these microorganisms is necessary in order to prevent the occurrence of foodborne diseases and when outburst of the disease appears, to reduce the spreading. It is important to develop strategies to ensure a safe food supply [4,5].
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