Micrococcus luteus: A Potential Keratinolytic Bacteria Isolated from Poultry Waste Dumping Site at Namakkal District, Tamil Nadu, India

Abstract

: In this study, the experimental soil samples were collected from poultry waste dumping site at Namakkal district, Tamil Nadu, India. From the five soil samples, 15 bacterial colonies were isolated based on Keratinolytic activity with source of agar nutrient medium, nitrogen, carbon and feather meal powder. After the incubation period only 6 isolates showed clear zone of hydrolysis around colony in feather meal agar medium incubated at 37 C for 72 h. The Bacterial species were identified by the morphological and biochemical tests compared with Bergey’s manual of systematic bacteriology IV edition. The obtained bacterial genera from poultry waste dumping sites were Escherichia coli, Enterococcus faecalis, Micrococcus luteus, Klebsiella pneumoniae, Proteus sp., Staphylococcus aureus, Salmonella sp. and Pseudomonas aeruginosa. During the Keratinolytic activity the maximum zone of inhibition was noticed in the isolate No.3, and it was identified as Micrococcus luteus. The identified potential keratinolytic bacterial species culture was optimized with different temperature, pH, nitrogen and carbon sources for bulk production of enzyme. The bulk production of enzyme was precipitated with 20% of ammonium sulphate and it was purified with dialysis bag and subjected to assay procedure for determination of enzyme activity. The antibiotic sensitivity was tested on the isolated bacterial isolates and the highest antibiotic resistance was observed in Staphylococcus aureus (75%) and second most Salmonella (70%) and followed by Klebsiella pneumoniae (60%), Enterococcus faecalis (50%) and Escherichia coli (45%) .