Abstract
A microchip interdigitated electrode with a sequential signal generator has been developed for traveling wave dielectrophoresis (twDEP) of biological cell suspensions. The electrode was fabricated on a microscope glass slide and coated with a 0.5 μm thickness of gold through a sputtering technique which was designed for large-scale inductions of cells rather than for individual cells as in previous versions of our device. As designed for a representative cell size of 10 μm, the electrode array was 50 μm in width to allow large numbers (>106) of cells to be processed. The sequential signal generator produces an arbitrary AC quadrature-phase to generate traveling electric field for a microchip interdigitated electrode. Each phase signal can be automatically altered and alternated with the other phases within interval time of 0.01-30 seconds (controlled by programming). We demonstrate the system could be used to estimate the dielectric properties of the yeast Saccharomyces cerivisiae TISTR 5088, the green alga Tetraselmis sp. and human red blood cells (HRBCs) through curve-fitting of dielectro- phoretic velocities and critical frequencies.
Highlights
It is well known that a micro-interdigitated electrode can be employed for dielectrophoresis (DEP) and traveling wave dielectrophoresis of cell suspensions [1,2,3,4]
A microchip interdigitated electrode with a sequential signal generator has been developed for traveling wave dielectrophoresis of biological cell suspensions
A microchip of two array-interdigitated electrode equipped with a sequential signal generator was developed for a study of traveling wave dielectrophoresis of biological cells
Summary
It is well known that a micro-interdigitated electrode can be employed for dielectrophoresis (DEP) and traveling wave dielectrophoresis (twDEP) of cell suspensions [1,2,3,4]. The microelectrodes for such work can generally be classified as three types: a one planar linear interdigitated array [1,5,6,7], two parallel arrays [2,3,4,8,9] and a multi-concentric ring structure [10]. Trode of two parallel arrays equipped with a sequential signal generator for experiments on traveling wave dielectrophoresis (twDEP) of cell suspensions. This microelectrode is designed for large-scale inductions of cells rather than for individual cells in our previous versions of the device such as “the octa-pair interdigitated electrode” described by [11]. Our newly developed system is a significant step towards biotechnology applications of the technology
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