Abstract
The synthesis of triglycerides by oral isolates and related type strains of Gram-positive bacteria was confirmed. The methodology involved a mild, specific trans-esterification of material recovered from thin-layer chromatograms of the neutral lipid fraction of bacteria grown on synthetic medium, followed by butylation of the liberated glycerol to yield tributyrin. Not only was quantitation of triglyceride content on the basis of gas-liquid chromatography of tributyrin feasible, but the simultaneous direct quantitation and identification of the individual fatty acids derived from the triglyceride was possible. The molar ratio of fatty acid to glycerol was calculated as additional proof of the presence of triglycerides.
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