Abstract
Extruded dry dog food products claimed to have a probiotic ingredient of Enterococcus faecium (NCIMB10415) in the commercial available formulations under the brand name of ProBiotic LIVE (Bacterfield S.A., Luxembourg) were studied in the present work using a multichannel thermal activity monitor TAM III. Maximum specific growth rate, heat produced during different growth phases, and lag-phase duration were determined. The length of the lag-phase that can be used to determine the time necessary for the probiotic ingredient to restore its activity after consumption of probiotic containing extruded products was also measured. The calorimetric data confirmed the ability of the Enterococcus faecium to grow at the acidic pH conditions, modeling conditions of gastro-intestinal tract of dogs, and preserve its metabolic activity (viability) at the same level as at the neutral pH. The results obtained indicated that microcalorimetry was a precise and convenient tool for monitoring probiotic activity in complicated solid-state matrices.
Highlights
Various attempts have been made to produce extruded feed or food product(s) with stable and viable probiotic bacteria count over the entire shelf-life period of the carrier product [1,2,3]
Extruded dry dog food products claimed to have a probiotic ingredient of Enterococcus faecium (NCIMB10415) in the commercial available formulations under the brand name of ProBiotic LIVE (Bacterfield S.A., Luxembourg) were studied in the present work using a multichannel thermal activity monitor TAM III
Prolongation of the lag-phase at low pH can be explained by acidic stress effect on the probiotic bacteria
Summary
Various attempts have been made to produce extruded feed or food product(s) with stable and viable probiotic bacteria count over the entire shelf-life period of the carrier product [1,2,3]. Very sensitive measurements of heat flows using microcalorimetry is one of the most attractive techniques for studying and monitoring of the metabolic activity of bacteria in opaque liquid media, and solid matrices [11,12,13]. It could be successfully applied in the studies of spoilage processes and shelf-life determination of products containing microbes [8,14,15,16], in all cases where the possibilities of optical and other physical methods are limited due to the non-transparency of media [17,18].
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