Microbiota of the rhizosphere zone of Calamagrostis epigeios from a coal mine waste dump

Abstract

The microbiota plays an important role in the processes of plant overgrowth of coal mine waste dumps, enabling the transformation of numerous compounds into forms available to plants. The overgrowth of coal mine dumps is influenced by many factors. Pioneers are plant species that have a wide ecological and phytocenotic amplitude. Calamagrostis epigeios (L.) Roth. occupies a special place among them. The composition of the microbiota of the rhizosphere zone of C. epigeios was studied in relation to the age of the plants and the stage of the succession of the “Vizeyska” mine dump (Ukraine). It was established and confirmed as a result of two-factor variance analysis that the growth phase of C. epigeios and the stage of the succession of coal mine waste dumps have different effects on the number of microorganisms from the rhizosphere zone of plants. The number of pedotrophic microorganisms, microorganisms that metabolize nitrogen of organic compounds, cellulose-degrading microorganisms, and microscopic fungi depended more on the age of C. epigeios, and not on the stage of the succession of the studied area. The number of chemolithotrophic bacteria, particularly thiobacteria, decreased with the change of the growth phase of C. epigeios. The number of acidophobic thiobacteria depended more on the stage of succession, and the number of acidophilic thiobacteria depended more on the age of the C. epigeios. The number of microorganisms that metabolize nitrogen of organic compounds, oligonitrophilic microorganisms and microorganisms that metabolize nitrogen of inorganic compounds in the samples of tailings from the area with grasses and perennials and from the area with grasses, shrubs, and sun-loving trees was higher, compared to the number of these groups of microorganisms in the control and changed with the change in the growth phase of C. epigeios. The number of microorganisms that metabolize nitrogen of organic compounds, oligonitrophilic microrganisms and microorganisms that metabolize nitrogen of inorganic compounds was the highest in the samples from the area with grasses, shrubs, and sun-loving trees during the adult growth phase of C. epigeios. In the area where C. epigeios dominated within the vegetation, the highest number of microorganisms that metabolize nitrogen of organic compounds was also during the adult phase of C. epigeios, and the number of bacteria that assimilate mineral forms of nitrogen and oligonitrophilic microorganisms was the highest during the sub-adult stage. The index of pedotrophicity is higher in the samples taken in the area where C. epigeios prevails among other herbaceous plants, and where in the tree layer there are Betula pendula, Populus tremula with an admixture of Pinus sylvestris. Pedotrophicity indices which were calculated for these samples do not depend on the growth stage of C. epigeios and are higher than for the control area. Immobilization-mobilization of nitrogen indices in samples of tailings from the area with grasses and perennials and from the area with grasses, shrubs, and sun-loving trees ranged from 1.94 to 3.52 and were higher compared to the control site.