Microbiota of Cow's Milk with Udder Pathologies.

Mariya V Gryaznova,Vitaliy I Zimnikov,Vitaliy I Mikhalev,Nikolay T Klimov,Sergey A Solodskikh,Mikhail Y Syromyatnikov,Evgeniy V Mikhaylov,Vasily N Popov,Yulia D Dvoretskaya

doi:10.3390/microorganisms9091974

Abstract

Mastitis is the most common disease for cattle, causing great economic losses for the global dairy industry. Recent studies indicate the multi-agent and microbiome diversity of this disease. To understand the nature of mastitis and investigate the role of the microbiome in the development of pathologies in the udder of bovines, we performed NGS sequencing of the 16S rRNA gene of cow’s milk with pathologies of the udder. The obtained data show a significant increase in the Cutibacterium, Blautia, Clostridium sensu stricto 2, Staphylococcus, Streptococcus and Microbacterium genera for groups of cows with udder pathologies. Increasing relative abundance of the Staphylococcus and Streptococcus genera was associated with subclinical mastitis. Our data show that a relative increase in abundance of the Staphylococcus and Microbacterium genera may be an early sign of infection. We have shown, for the first time, an increase in the Colidextribacter, Paeniclostridium and Turicibacter genera in groups of cows with mastitis. These results expand our understanding of the role of the microbiome in the development of bovine mastitis.

Highlights

IntroductionPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
In the group with acute catarrhal mastitis, we observed a significant increase in the Cutibacterium genus in comparison with the control group
Our data show that the milk microbiome of cows with udder pathologies differs significantly from the milk microbiome of healthy cows

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Mastitis is the most common inflammatory disease of the mammary gland of cattle, detected by an increase in the number of somatic cells (SCC) or visible abnormalities in milk [1]. The clinical manifestation is characterized by physical changes in the gland, which lead to a deterioration in the quality of milk and a decrease in its volume [2]. In subclinical mastitis, visual symptoms and signs are absent, but there is an increase in milk

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