Microbiota from &amp;lt;i&amp;gt;Helix aspersa&amp;lt;/i&amp;gt; M&amp;#252;ller in Barcelona Area (Spain)

Lucia Pérez Caullan,Agustín Calvo,Gisela Girmé Vila,Esteban Arosemena Angulo,M Angeles Calvo Torras,José Antonio Marcelo

doi:10.4236/aim.2014.410066

Abstract

In recent decades snail consumption has been increasing throughout Europe. The Heliciculture meets this need and gets snail farms, in order to get consistent production in the shortest possible time. The research provided, has a main objective to establish the microbiota of Helix aspersa Müller, both from healthy animals, and individuals with gastrointestinal processes. The samples selected for the study were feces obtained from snails collected in both breeding farms as field snails. In the case, farm animals were selected apparently form healthy individuals and others were with signs of gastrointestinal process study. Aliquots of each sample were plated on a range of culture media and incubated under different conditions of temperature and respiration. From the results we can note that the healthy snails (farmed or free-living) have been isolated and identified mostly, as strains belonging to the genera Pediococcus, Lactobacillus and Lactococcus and five strains of yeasts of the genera Candida and Cryptococcus. Individuals suffering from gastrointestinal disturbances processes have been isolated in high proportion species of Klebsiella, Pantoea, Citrobacter and Enterobacter. The results obtained indicate that the snails with health problems reduce the presence of strains of Lactobacillus and Lactococcus in favor of increasing and/or establishing colonization by strains of Enterobacteriaceae.

Highlights

The snail is part of the general situation of agricultural activity, which is defined as “full cycle raising edible terrestrial snails.” This is a word composed of “Helici”, Helix derived from the name given to a genus of its shellHelix aspersa Müller is called common snail or Bovë, depending on the area of Europe
From Man Rogosa Sharpe (MRS) agar isolation were obtained 17 strains that showed morphologies and characteristics of lactic acid bacteria, of which 11 strains belong to the genera Pediococcus, Lactococcus and Lactobacillus (See Table 1)
The genera Pediococcus and Lacobacillus were isolate in a 27.27% corresponding to the specie Pediococcus pentosaceus, Lactoccocus lactis and Lactobacillus was isolate in a 45.45% corresponding to a species: Lactobacillus plantarum, Lactobacillus brevis and Lactobacillus acidophilus

Summary

Introduction

The snail is part of the general situation of agricultural activity, which is defined as “full cycle raising edible terrestrial snails.” This is a word composed of “Helici”, Helix derived from the name given to a genus of its shellHelix aspersa Müller is called common snail or Bovë, depending on the area of Europe. The snail is part of the general situation of agricultural activity, which is defined as “full cycle raising edible terrestrial snails.”. This is a word composed of “Helici”, Helix derived from the name given to a genus of its shell. Helix aspersa Müller is called common snail or Bovë, depending on the area of Europe. This variety is used on farms dedicated to raising snails for being the most popular in European countries where it is consumed by the snail, especially in France and Spain. Wild snails with food and commercial interest, generate a whole agri-food production sector called “helicícola industry”, which is linked to the rural implications that are majorly environmental, economic and cultural, including industries and downstream activities, such as preparation, processing, marketing and consumption of the product [4]-[8]

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Results

Conclusion