Abstract
Thelephora ganbajun is a wild edible mushroom highly appreciated throughout China. The microbiomes of some fungal sporocarps have been studied, however, their potential functional roles currently remain uncharacterized. Here, functional gene microarrays (GeoChip 5.0) and amplicon sequencing were employed to define the taxonomic and functional attributes within three micro-niches of T. ganbajun. The diversity and composition of bacterial taxa and their functional genes differed significantly (p < 0.01) among the compartments. Among 31,117 functional genes detected, some were exclusively recorded in one sporocarp compartment: 1,334 genes involved in carbon (mdh) and nitrogen fixation (nifH) in the context; 524 genes influencing carbon (apu) and sulfite reduction (dsrB, dsra) in the hymenophore; and 255 genes involved in sulfur oxidation (soxB and soxC) and polyphosphate degradation (ppx) in the pileipellis. These results shed light on a previously unknown microbiome and functional gene partitioning in sporome compartments of Basidiomycota. This also has great implications for their potential ecological and biogeochemical functions, demonstrating a higher genomic complexity than previously thought.
Highlights
Basidiomycota, which includes the mushroom-forming fungi and related taxa, is one of two large divisions within the Fungi Kingdom that, together with Ascomycota, constitute the subkingdom Dikarya
A total of 280,503 16S sequences for bacterial community were obtained from the three T. ganbajun compartments
The highest bacterial diversity was found in the context (C) and the lowest in the hymenophore (H)
Summary
Basidiomycota, which includes the mushroom-forming fungi and related taxa, is one of two large divisions within the Fungi Kingdom that, together with Ascomycota, constitute the subkingdom Dikarya (often referred to as “higher fungi”). Basidiomycota is a structural and functional component of paramount importance in earth ecosystems as either saprotrophs, pathogens or ectomycorrhizal fungi. They are culturally significant as food, Microbiome Community in Basidioma Microniches medicine, drugs and spiritual symbols, with socioeconomic relevance since early human history (Pérez-Moreno et al, 2020). Mushrooms including those from Basidiomycota are currently a main income source for millions of indigenous farmers across the world (FAO, 1997)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
