Abstract

The corrosion of 304 stainless steel by Bacillus subtilis was investigated under different glycerol (electron donor) and nitrate (electron acceptor) concentrations. When nitrate was deficient, the passive film served as an alternative electron acceptor to complete outward extracellular electron transfer (EET) of B. subtilis, inducing more severe corrosion especially in the early stage. When glycerol concentration was deficient, the inward EET of B. subtilis was significantly enhanced, resulting in accelerated corrosion in the late stage. These results demonstrated that bidirectional EET was involved in the corrosion of stainless steel by B. subtilis and depended on the electron donor/acceptor concentrations.

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