Microbiological synthesis of L-cysteine and its Analogues. Part II. Synthesis of L-cysteine and its analogues by intact cells containing cysteine desulfhydrase.

Abstract

Cysteine desulfhydrase catalyzes β-replacement, the reverse reaction of α, β-elimination, as well as a, β-elimination. These reactions were studied with intact cells of Aerobacter aerogenes 1-3-2 and Aerobacter cloacae IFO 12009 containing cysteine desulfhydrase. L-Cysteine and its analogues were synthesized by replacement and reverse reactions using intact cells. β-Chloro-L-alanine, L-cysteine, S-methyl-L-cysteine, S-allyl-L-cysteine and L-serine were used as substrates together with hydrogen sulfide and methyl mercaptan to synthesize L-cysteine and S-methyl-L-cysteine via replacement reaction by intact cells. L-Cysteine synthesized from β-chloro-L-alanine was confirmed to be entirely in L-form after isolation and identification of the product. The reverse reaction for synthesis of L-cysteine and S-methyl-L-cysteine from hydrogen sulfide or methyl mercaptan, pyruvate and ammonia was also catalyzed by intact cells. β-Chloro-L-alanine was found to be the best substrate for synthesis of L-cysteine and S-methyl-L-cysteine by β-replacement reaction.