Microbiological study of vaginitis among women of reproductive age group

Abstract

: Vaginitis is the most common health problem among women of reproductive age group, which is associated with gynaecological and obstetrical complications and also increases the transmission of HIV and other sexually transmitted diseases.: This study aimed at estimating the various etiological agents causing vaginitis, different laboratory methods for its diagnosis, isolation of different species and antibiotic susceptibility pattern of aerobic bacterial isolates.: In present study, 104 clinically suspected cases of vaginitis attending gynaecology OPD was included after obtaining informed consent and various data were collected using via questionnaire. Three high vaginal swabs collected from each patient and subjected to PH, Whiff test, Microscopic examination (Grams staining, KOH mount, Acridine orange staining (AO), Saline wet mount) Culture and Biochemical reactions following standard protocols. Aerobic isolates were subjected to antibiotic susceptibility testing as per CLSI guidelines.: Out of 104 Clinically suspected cases most common type of vaginitis were Bacterial vaginosis (BV)51.9% followed by Candidiasis 41.3%, Trichomoniasis 3.9%. Most common species isolated in vulvovaginal Candidiasis is 32.5%. Majority of Gram positive organisms were susceptible to Linezolid and Gram negative organisms to Azithromycin.: Bacterial vaginosis, Candidiasis and Trichomoniasis are common problem in women of reproductive age, therefore screening of vaginal infection in women of reproductive age should be implemented. Culture and sensitivity should be mandatory and treatment should be based on invitro susceptibility testing. So that misuse of antibiotics will be avoided.