Microbiological Quality Assessment of Skin and Body care Cosmetics by using Challenge test

Abstract

Cosmetic products may be exposed to microbial contamination during storage or transport, and to avoid the risk of microbial growth, manufacturers add preservative compounds as a protection for the product from spoilage. The Microbial Challenge test is a procedure to evaluate the preservative efficacy by challenging the product with testing microorganisms to determine the quality of preservation. In this study, thirty-two cosmetics products used for body and skin care were collected from markets and pharmacies in Mecca region, these products are subjected to microbiological analysis, results show that most samples are contaminated except six samples. Non contaminated samples were subjected to European Pharmacopeia 7.0 standards. Results show that two samples, foaming gel and body and face cream are failed to demonstrate the required microbiocidal effect against the S.aureus test species, results recorded 1.21 x 105 and 6.80 x 105 (CFU/ml) respectively at the second day of incubation, other products: shower gel, hand wash, body lotion and shampoo demonstrate that required microbiocidal effect against the test species during day 2, 7, 14 until day 28th. The microbial count number is less than 10 during all incubation periods. To prevent contamination in cosmetics, manufacturers are required to add a good preservative system to the products and examine them before sale. Due to the high percentage of microbial contamination in cosmetics in Mecca region and for consumers safety, this study is prepared.