Abstract
A simple and precise microbiological method has been developed for the quantitative estimation of Cyanocobalamin in pharmaceutical formulations. After employing different microorganisms for achieving best results, Escherichia coli 113-3 ATCC No. 11105 was found to be the most suitable. In the method zone of exhibition achieved by using the aforesaid organism were measured from which content of Cyanocobalamin in pharmaceutical formulation was determined. The results were good in most of the formulations available in the market. Cyanocobalamin is added in 100% overages in the pharmaceutical formulations which were shown during its estimation. Moreover there is no interference of other vitamins present in the formulations by using this culture & method. The method employed is economical and simple and can be used in the quality control of bulk manufacturing and also in pharmaceutical formulations
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