Microbiological indicators from sputum of patients with exacerbation of COPD

Abstract

Objectives: Rational antibiotic therapy in exacerbation of COPD should be based on local data of antibiotic susceptibility major respiratory pathogens. The aim of the study – to determine microbial landscape and antibiotic susceptibility testing major pathogens from sputum of patients with exacerbation of COPD. Methods: A total of 370 strains isolated from sputum of 267 hospitalized patients with exacerbation of COPD at National Scientific Medical Research Center during 2010 – 2014 were included to the study. The identification of isolates and antibiotics susceptibility testing were performed by VITEK 2 (bioMerieux) automated system. Results: Analyses of microbial landscape of sputum showed that 66.4% isolates belongs to the Streptococcus. Exactly Streptococcus pneumoniae was the most frequent pathogen from sputum of patients with exacerbation of COPD (38.2% from total isolates). Further, the frequency of inoculation was: Moraxella catarrhalis – 11.6%, Enterobacteriaceae – 4.3%, Pseudomonas aeruginosa – 3.0%, Staphylococcus aureus – 2.7%. increasing resistance Streptococcus pneumoniae: to erythromycin from 56.2% in 2010 to 85.0% in 2014, to penicillin from 20.6% to 31.8%, to levofloxacin from 10.3% to 36.3%, respectively. Conclusion: Monitoring the microbial landscape sputum of patients with exacerbation of COPD showed that Streptococcus pneumoniae was the main respiratory pathogens with increasing resistance to beta-lactams, quinolones and macrolides.