Microbiological Diagnoses on Clinical Mastitis-Comparison between Diagnoses Made in Veterinary Clinics versus in Laboratory Applying MALDI-TOF MS.

Lærke Boye Astrup,Michael Farre,Karl Pedersen

doi:10.3390/antibiotics11020271

Lærke Boye Astrup, Michael Farre + Show 1 more

Open Access

https://doi.org/10.3390/antibiotics11020271

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Abstract

The present study compares the diagnoses on clinical bovine mastitis made in veterinary clinics using conventional diagnostic methods with diagnoses on the same samples made by a veterinary reference laboratory using MALDI-TOF MS as diagnostics. The study enables targeted and evidence-based consulting on prudent mastitis diagnostics and related antibiotic usage. In total, 492 samples from clinical mastitis were included. When applying MALDI-TOF MS as gold standard, only 90 out of 492 diagnoses made in veterinary clinics, equal to 18%, were correct. Four main findings were important: (1) the veterinary clinics overlooked contamination in mastitis samples; (2) the veterinary clinics only assigned 2 fully correct diagnoses out of 119 samples with mixed growth cultures; (3) the veterinary clinics made close to half of their diagnoses on pure culture erroneously; (4) the veterinary clinics applied a limited number of the relevant pathogen identifications on pure culture samples. Altogether, the present study shows that a large part of Danish clinical mastitis cases are misdiagnosed. Lack of correct diagnoses and diagnostic quality control may lead to the choice of wrong treatment and thus hamper prudent use of antibiotics. Hence, the present study warns a risk of overuse of antibiotics in Denmark. Consequently, the present study calls for training of veterinary clinics in diagnostics of mastitis pathogens and national guidelines on quality assurance of mastitis diagnostics.

Highlights

Mastitis is one of the most frequent and costly diseases in dairy cattle [1,2]
Results on pure culture samples: The MALDI-TOF MS analysis identified 147 pure cultures out of the 492 samples provided by the veterinary clinics
Based on the accepted pooling of pathogens, the MALDI-TOF MS confirmed the pathogen identification assigned by the veterinary clinics in 77 out of the 132 pure cultures

Summary

Introduction

Mastitis is one of the most frequent and costly diseases in dairy cattle [1,2]. Mastitis accounts for a large part of antibiotic consumption in adult dairy cattle [3,4] and compromises animal welfare [5]. Since the early 1980s, most Danish veterinary clinics obtain their diagnoses based on inhouse microbiological analysis. These analyses are performed without any formal quality assurance or accreditation. The applied analyses include Gram-stain or potassium hydroxide test, catalase test, coagulase test, microscopy and morphological characterization based on both blood agar and CHROMagarTM Orientation or other selective and indicative agars. Such analyses results in a limited set of phenotypic biochemical characteristics and do not appropriately differentiate the variety of species that can be present in milk and will grow on blood agar. These limitations have become more and more evident with the gains of molecular techniques, disguising the extensive variety of mastitis-causing pathogens [7,13,14,15,16]

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