Abstract
Abstract Studies of the microbial assay of streptomycin were conducted to evaluate (1) four methods of preparing compensating standard curves and (2) a n IRC-50 resin separation method to overcome the interference of feed components in low level feeds. Compensating curves were prepared from extracts of blank feed, from extracts of feed in which streptomycin was adsorbed by IRC-50 in a batch method and also in a column method, and from extracts of feed by the method of standard addition. Recoveries of streptomycin using the extract of blank feed and extract of feed by the method of standard addition were promising (83–96%), whereas recoveries using IRC-50 resin-inactivated extract were low (47–62%). The IRC-50 resin separation method was found to be satisfactory in principle, but the interference of the feed components was not resolved. Further studies are planned on the compensation methods and the resin separation method.
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