Abstract
Abstract Modifications of the official AOAC method for the determination of streptomycin in feeds were collaboratively studied. The cylinder plate assay uses Bacillus subtilis ATCC 6633 as the test organism; low levels of streptomycin are determined with sulfadiazine-sensitized assay agar and high levels are determined with nonsensitized assay agar. The modifications include a single extraction and either mechanical shaker or high-speed blender as alternative methods of extraction. Four feed levels at 6 to 75 g streptomycin/ton were analyzed by the sensitized method. The average recoveries ranged from 81 to 98% and the coefficients of variation ranged from 4.5 to 9.7%. Also, four feed levels containing 37.5 to 300 g streptomycin/ton were analyzed by the non-sensitized assay. The spread of recoveries among 18 collaborators was too wide for this method to be acceptable at the levels studied. It is recommended that the sulfadiazine-sensitized assay be adopted as official first action at 6 g/ton and that the non-sensitized assay be approved at the level of 2.8 g/lb and study continued at lower levels.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
